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     1  ~ 2014 oct 03custom peptide synthesisCustom Peptide Synthesis Service - Pierce Biotechnology, Inc.Thermo Scientific Custom Peptide Synthesis Service provides synthetic peptides with a wide range of labels, modifications, scales, purities and delivery time ...
     1  ~ 2014 sep 24brijsolutionBrij 35 Detergent Solutions - Pierce Biotechnology, Inc.Stabilized Brij 35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.
     1  ~ 2014 sep 20piercePierce Protein Biology - purify, modify and detect proteinsStudy proteins in their cellular context - detect, measure and explore protein expression, identity and function using Thermo Scientific Pierce Protein Biology ... ‎Product Catalog - ‎Contact Us - ‎Protein Assays and Quantitation - ‎Antibodies
     1  ~ 2014 sep 13spectra multicolorSpectra Multicolor Broad Range Protein LadderThermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use in gel ...
     1  +1 2014 sep 08immunoprecipitation actin contaminationCo- Immunoprecipitation (Co-IP) - Pierce Biotechnology, Inc.A review of co- immunoprecipitation as a protocol to study protein-protein ... nonspecific binding to IP components and antibody contamination that may mask ... target complex is likely to occur, especially with abundant proteins such as actin .
     1  +1 2014 sep 03elisa platesELISA Reagents, Microplates and AccessoriesSubstrates, buffers, plates and various other supplies to develop and complete ELISA and EIA experiments; includes high-binding capacity coated microplates ...
     1  +1 2014 aug 27protein purification columnsProtein Affinity Purification - PierceAgarose beads and other resins for specific purification of proteins and other target ... for covalent immobilization of ligands, and columns for packing affinity resins.
     1  ~ 2014 aug 25tmb peroxidase substrateTMB Substrates - PierceThermo Scientific Pierce TMB ELISA Substrates detect horseradish peroxidase ( HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to ...
     1  +14 2014 aug 22peptide customer listCustom Peptide Synthesis Service - PierceThis service includes advanced custom peptide capabilities with a wide ... by MS alone or combined with analytical HPLC; Flexible – extensive list of available ...
     1  ~ 2014 aug 22pvdf transfer membranePVDF Membranes - PierceWestern blotting – PVDF transfer membranes manufactured especially for protein transfer and Western blot applications; Compatible and durable – polyvinyl ...
     1  ~ 2014 aug 18gst pull down assayPull - Down Assays - Pierce Biotechnology, Inc.A review of pull - down assay methodology, including the different choices in protocol design (tag or binding domain), tags (biotin, GST , etc), information for ...
     1  ~ 2014 aug 18his-tagged protein purificationHis - tagged Proteins – Production and Purification - Pierce A review of the histidine ( His ) tag for protein purification using metal (cobalt, nickel or copper)-chelated agarose or sepharose beads in pulldown or ...
     1  +2 2014 aug 15custom peptide email listThis service includes advanced custom peptide capabilities with a wide range of labels, modifications,
     1  ~ 2014 aug 14gst pull downPull - Down Assays - Pierce
     1  ~ 2014 aug 14protein a affinity columnOverview of Affinity Purification - PierceProteins and other macromolecules of interest can be purified from crude extracts or other complex ... ‎Introduction - ‎Affinity Chromatography vs ... - ‎Supports for Affinity Purification
     1  ~ 2014 aug 02detection limit western blotGuide to Chemiluminescent Western Blot Substrates - PierceChoose an appropriate ECL reagent for Western blot detection . ... ease-of-use, sensitivity (i.e., lower limit of detection ) and compatibility with imaging equipment.
     1  ~ 2014 aug 02recombinant protein aPierce Recombinant Protein A and ConjugatesPurified and peroxidase-conjugated recombinant Protein A to detect rabbit and human antibodies, especially IgG isotypes, in Western blot, ELISA and other ...
     1  ~ 2014 jul 30protein a agarose beadsProtein A Agarose - PierceStandard-capacity, immobilized native Protein A beaded agarose resin for a ... The agarose beads have physical and chemical properties that enable them to be ...
     1  ~ 2014 jul 30production of specific antibodyAntibody Production (Immunogen Preparation) - PierceThe production of specific antibody probes is a relatively straightforward process involving immunization of animals and reliance upon their immune systems to ...
     1  ~ 2014 jul 29ecl western blot32106 - Pierce ECL Western Blotting SubstrateThermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry- level peroxidase substrate for enhanced chemiluminescence (ECL) that directly ...
     1  ~ 2014 jul 20cells hard to lyseOverview of Cell Lysis and Protein Extraction - PiercePlant cell walls are particularly strong, making them very difficult to disrupt mechanically or chemically. Until recently, efficient lysis of yeast cells required ...
     2  -1 2014 sep 29ultra lowrange protein ladderSpectra Multicolor Low Range Protein LadderThermo Scientific Spectra Multicolor Low Range Protein Ladder is a mixture of six (6) proteins and synthetic peptides (1.7 to 40kDa) for use as size standards for  ...
     2  ~ 2014 sep 24bperB-PER Protein Extraction Reagents - Pierce Biotechnology, Inc.Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or ...
     2  ~ 2014 sep 22zeba glycerolGlycoLink™ Micro Immobilization Kit - Pierce Biotechnology, Inc.Zeba ™ Spin Desalting Columns, 7K MWCO, 10 × 0.5mL ... Primary amines (e.g., Tris, glycine), glycerol and reducing agents in the antibody solution will ...
     2  ~ 2014 sep 17cross linkerCrosslinker Selection Guide - Pierce Biotechnology, Inc.This Crosslinker Selection Guide provides quick access to customized lists of Thermo Scientific Pierce Crosslinkers that meet specific criteria, including target ...
     2  +1 2014 sep 15temedTetramethylethylenediamine ( TEMED ) - Pierce Biotechnology, Inc.Thermo Scientific Pierce Tetramethylethylenediamine ( TEMED ) is an essential catalyst for polyacrylamide gel polymerization. TEMED is used with ammonium ...
     2  ~ 2014 sep 14fermentas protein ladderPageRuler Plus Prestained Protein Ladder 10-250KThermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250kDa) for use as size ...
     2  ~ 2014 sep 0740 tips and techTech Tips | Pierce Previews - Pierce Biotechnology, Inc.Index menu of printable protocols and other technical tips for methods involving ... Tech Tip # 40 · Convert between times gravity (x g) and rotor speed (RPM) ...
     2  ~ 2014 sep 07recombinant protein expressionOverview of Protein Expression Systems - Pierce Biotechnology, Inc.In general, proteomics research involves investigating any aspect of a protein such as structure, ... ‎Introduction to Protein Expression - ‎Transcription - ‎Translation
     2  ~ 2014 sep 05lysis protein purificationOverview of Cell Lysis and Protein ExtractionA review of cell lysis and protein extraction that emphasizes buffers and ... step in cell fractionation, organelle isolation and protein extraction and purification .
     2  ~ 2014 sep 03what is cross linkingChemistry of Crosslinking - Pierce Biotechnology, Inc.A review of of the chemistry of the reactive groups of heterobifunctional and homobifunctional crosslinking reagents, including amine, sulfhydryl, carboxyl ...
     2  ~ 2014 aug 30luciferase assay kitPierce Firefly Luciferase Glow Assay KitThe Thermo Scientific Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the ...
     2  ~ 2014 aug 26protein purification column
     2  +1 2014 aug 17protein protein interactionOverview of Protein - Protein Interaction AnalysisA review of the nature of protein - protein interactions , the types of interactions and a description of common methods and assays of detection. (07a-a-Protein ...
     2  +55 2014 aug 07monoclonal antibodies classAntibody Structure and Classes - PierceA review of the various types of antibodies, including the structures and ... on polyclonal and monoclonal antibodies (11-f-Antibody Structure and Class ).
     2  +1 2014 jul 30custom peptide synthesis price
     2  ~ 2014 jul 25bacterial cell lysisTraditional Methods of Cell Lysis - PierceA review of techniques to physically lyse cells, including homogenization, ... French press is often the method of choice for breaking bacterial cells mechanically.
     2  ~ 2014 jul 17igg molecular weightEach heavy chain has about twice the number of amino acids and molecular weight (~50,000) as each light chain (~25,000), resulting in a total immunoglobulin  ...
     3  +98 2014 sep 28kits de elisaELISA Kits - Pierce Biotechnology, Inc.96-well plate sandwich ELISA kits and matched antibody pairs to assay cytokines , growth factors and soluble receptors in serum, plasma, ...
     3  ~ 2014 sep 16triton x 100Triton X - 100 Detergent Solution - Pierce Biotechnology, Inc.Thermo Scientific Surfact-Amps X - 100 Detergent Solution is highly-purified Triton ™ X - 100 detergent stabilized as a 10% solution in 10mL glass ampules or ...
     3  +1 2014 sep 12kit conjugation antibodiesProtein and Antibody Labeling - Pierce Biotechnology, Inc.Labeling reagents and kits to label an antibody or other protein to make a fluorescent probe, enzyme conjugate , biotinylated binding reagent or iodine labeled ...
     3  +1 2014 sep 11tio2TiO2 Phosphopeptide Enrichment and Clean-upThermo Scientific Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit enables fast, selective enrichment of phosphorylated peptides for mass ...
     3  +3 2014 sep 06edta free shippingHalt Protease Inhibitor Cocktail, EDTA - Free (100X) - Pierce Regular and EDTA - free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively. The stable, concentrated  ...
     3  -1 2014 aug 30sds molecular weightSDS ( Sodium Dodecyl Sulfate -Lauryl) - PierceStandard-grade SDS detergent popular for routine protein electrophoresis and cell ... Molecular Weight : 288.5g; Detergent Class: Ionic (anionic); Aggregation ...
     3  -1 2014 aug 26western blot analysisOverview of Western Blotting - PierceA review of the Western blotting protocol that discusses protein separation and ... by Towbin, et al. in 1979 and is now a routine technique for protein analysis .
     3  -2 2014 aug 26gst tag sequenceGST - tagged Proteins - PierceA review of glutatione S-transferase (GST) as a fusion tag for the purification of ... The result of expression from this vector is a GST - tagged fusion protein in ...
     3  +1 2014 aug 23beta galactosidase assaybeta - Galactosidase Assay Kit - PierceThe Thermo Scientific Mammalian β- Galactosidase Assay Kit provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta -Gal  ...
     3  ~ 2014 aug 11dialysis membrane pore sizeSeparation characteristics of dialysis membranes | Pierce PreviewsThe separation characteristic determined by the pore size -range of a dialysis membrane is most often referred to as the molecular weight-cutoff ...
     3  -1 2014 jul 29bsa standard curveBovine Serum Albumin ( BSA ) Standards - PierceHigh-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.
     3  +1 2014 jul 26endotoxin lal test88282 - Pierce LAL Chromogenic Endotoxin Quantitation KitThe endotoxin concentration in a sample is measured using the Pierce LAL ... 50- test kit, Product Instructions for product #88282 Pierce LAL Chromogenic ...
     3  ~ 2014 jul 24cardiomyocytes rat buyOne-hour procedure to isolate primary cardiomyocytes from Isolating primary cardiomyocytes from neonatal mouse and rat hearts has typically been a time-consuming, labor-intensive task. A common ...
     4  +97 2014 sep 27proteinosa kProteinase K - Pierce Biotechnology, Inc.Thermo Scientific Pierce Proteinase K is a protease that cleaves at the carboxyl side of aliphatic, aromatic or hydrophobic residues and is commonly used to ...
     4  +1 2014 sep 19mes bufferMES Buffered Saline Packs - Pierce Biotechnology, Inc.Thermo Scientific BupH MES Buffered Saline Packs are pouches of dry-blended powder that are each sufficient to make 500mL of optimized amine-free and ...
     4  +1 2014 aug 29bsa bovine serum albuminBovine Serum Albumin (BSA) Standards - PierceThese bovine serum albumin (BSA) solutions are protein concentration reference standards for use in BCA, Bradford and other protein assay protocols. BSA is ...
     4  -1 2014 aug 26western blot methodWhile there are many different tags that can be conjugated to a secondary or primary antibody, the detection method used ...
     4  ~ 2014 aug 26enzyme linked immunosorbent assayOverview of ELISA - PierceEnzyme - linked immunosorbent assays (ELISAs) are plate-based assays designed for detecting and quantifying substances such as peptides, proteins, ...
     4  ~ 2014 aug 21luciferase reporter assayLuciferase Reporter Assays - PierceLuciferase reporter reagents, multiple cloning site vectors, and flash, glow and dual-spectral luciferase assay kits based on spectrally resolvable Cypridina, ...
     4  +6 2014 aug 19antibodies are produced byOverview of Antibody Production and PurificationThe ability of animal immune systems to produce antibodies capable of binding specifically to antigens can be harnessed to manufacture probes for detection of  ...
     4  ~ 2014 aug 18neutral buffered formalinFixation Strategies and Formulations - Pierce Biotechnology, Inc.Physical Fixation; Formaldehyde, Glutaraldehyde and Other Chemical ... For example, 10% neutral - buffered formalin (NBF or simply formalin) is really a 4% ...
     4  +1 2014 aug 14bovine serum albumin
     4  +9 2014 aug 13flash detection kitPierce Firefly Luciferase Flash Assay KitThese reagents are optimized to work with Thermo Scientific Firefly Luc Plasmids ; however, the Flash Assay Kit may be used to detect the activity of other firefly ...
     4  ~ 2014 aug 11blue ray xCL-XPosure Film ( X - ray Film) - PierceThermo Scientific CL-XPosure Film is an economically priced clear- blue X - ray film especially for detection and documentation of ECL-based Western blots and  ...
     4  ~ 2014 aug 10sodium dodecyl sulfateThermo Scientific Sodium Dodecyl Sulfate (Lauryl) is standard-grade SDS detergent for use in protein polyacrylamide gel electrophoresis (PAGE).
     4  -1 2014 aug 01protein a purificationAgarose beads and other resins for specific purification of proteins and other target molecules, kits for immunoprecipitation (IP), activated resins for covalent ...
     4  ~ 2014 aug 01chemistry of proteinChemistry of Protein Assays - PierceA review of the chemistry of protein quantitation assays, including the copper- based BCA and modified Lowry assays and the dye-based Coomassie (Bradford ) ...
     4  +2 2014 jul 26rapid protein purification kitMelon Gel IgG Purification Kits - PierceRapidly purify human, mouse and rabbit IgG from serum and other sources with this simpler alternative to traditional Protein A and Protein G methods.
     4  -1 2014 jul 20sodium dodecyl sulphateSDS ( Sodium Dodecyl Sulfate -Lauryl) - Pierce Biotechnology, Inc.
     4  ~ 2014 jul 16cell lysis bufferPierce Cell Lysis Reagents for Protein ExtractionGentle whole cell lysis buffers for high-yield protein extraction and solubilization from E. coli and other bacteria, cultured mammalian cells and tissues, insect ...
     5  -1 2014 oct 04fmoc heavy amino acidsHeavyPeptide AQUA Custom Synthesis ServiceHeavyPeptide sequences up to 15 amino acids in length are synthesized using the latest Fmoc solid-phase technology and purified by HPLC. All HeavyPeptide  ...
     5  ~ 2014 oct 03polyclonal antibody productionPolyclonal antibodies are recovered directly from serum (bleeds). Monoclonal antibodies are produced by fusing antibody-secreting spleen cells from ...
     5  -1 2014 sep 27preis pbs pufferSuperBlock Blocking Buffers - Pierce Biotechnology, Inc.Thermo Scientific SuperBlock Blocking Buffer in PBS is better than milk for sensitive detection of target proteins. HeLa cell lysate (20, 10, 5, 2.5, 1.25, 0.625 and ...
     5  -2 2014 sep 27glutathion s transferaseGST-tagged Proteins - Pierce Biotechnology, Inc.Glutathione S - transferase (GST) is a 211 amino acid protein (26kDa) whose DNA sequence is frequently integrated into expression vectors for production of ...
     5  -3 2014 sep 25sh groupsSulfhydryl-reactive Crosslinker Chemistry - Pierce Biotechnology, Inc.Besides amine-reactive compounds, those having chemical groups that form bonds with sulfhydryls (– SH ) are the most common crosslinkers and modification  ...
     5  -1 2014 sep 25fitcFITC and Fluorescein Dyes and Labeling KitsFluorescein isothiocyanate ( FITC ) and NHS-Fluorescein are amine-reactive derivatives of fluorescein dye that have wide-ranging application as antibody and  ...
     5  +1 2014 sep 21thermo scientificStudy proteins in their cellular context - detect, measure and explore protein expression, identity and function using Thermo Scientific Pierce Protein Biology ...
     5  ~ 2014 sep 21bradford protein measurementCoomassie ( Bradford ) Protein Assay - Pierce Biotechnology, Inc.The Thermo Scientific Pierce Coomassie Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595nm)  ...
     5  -1 2014 sep 19lal test
     5  +1 2014 sep 18plus kitMem-PER Plus Membrane Protein Extraction KitThe Thermo Scientific Mem-PER Plus Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of integral membrane proteins and ...
     5  +84 2014 sep 11reagent review us historyTraut's Reagent - Pierce Biotechnology, Inc.2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary
     5  ~ 2014 aug 29what is elisa methodDetection Strategies for ELISA . The final stage in all ELISA systems is a detection step. Unless a radioactive or ...
     5  ~ 2014 aug 21poly ethylene glycolPolyethylene Glycol ( PEG ) and PEGylation - PierceA review of the use of polyethylene glycol ( PEG )in research applications, including a description of PEG properties and their use in crosslinkers and biotinylation ...
     5  -1 2014 aug 15cell viability assayalamarBlue Cell Viability Assay Reagent - PierceThe Thermo Scientific alamarBlue Cell Viability Assay Reagent incorporates a redox-sensitive growth indicator to quantitatively measure cell viability and ...
     5  -1 2014 jul 29solid phase peptide synthesisPeptide Synthesis - PierceA review of the applications and methodology of peptide synthesis , including details on solid phase and liquid phase synthesis , Fmoc and Boc protection ...
     5  -1 2014 jul 19gst protein buyGST -tagged Proteins - PierceA review of glutatione S-transferase ( GST ) as a fusion tag for the purification of proteins through column- and plate-based immunoprecipitation, ...
     6  +6 2014 oct 01mouse embryonic fibroblastsAn improved method for highly efficient isolation of primary mouse Mouse embryonic fibroblasts (MEFs) play a vital role in stem cell research where they function as feeder cells to maintain mouse and human ...
     6  +3 2014 aug 15lysozyme solution cell lysisLysozyme - PierceAlthough hen egg white lysozyme is most effective for the lysis of gram-positive ... Bacterial cell wall lytic enzyme that improves inclusion body protein ... Must be readily soluble at 5mg/mL in 0.1M KH2PO4 to give a clear, colorless solution .
     6  +3 2014 aug 01protein binding assayMethods for Detecting Protein -DNA Interactions - Pierce(EMSA). The EMSA is used to study proteins binding to known DNA oligonucleotide probes and can be used ...
     6  -2 2014 jul 28human igg elisa kitEasy-Titer Antibody Assay Kits - PierceDetermine antibody concentration in serum and culture supernatant with kits specific for mouse, rabbit, or human IgG , and human IgM.
     6  +2 2014 jul 26endotoxins peptide removalPierce High Capacity Endotoxin Removal ResinThermo Scientific Pierce High Capacity Endotoxin Removal Resin binds to reduce endotoxin levels in protein samples by ≥99% in 1 hour while maximizing  ...
     7  +6 2014 sep 13carbodiimide resinCarbodiimide Crosslinker Chemistry - Pierce Biotechnology, Inc.A review of EDC and DCC carbodiimide reagent chemistries for carboxylic acid ... an amine-derivatized surface material or solid support such as agarose resin .
     7  ~ 2014 aug 28linkers in literatureCrosslinking Reagents - PierceCrosslinker Applications Guide provides recent literature references for specific crosslinker and applications; Crosslinkers at a Glance provides a quick way to ...
     7  +15 2014 aug 27fermentas thermo fisherPageRuler Plus Prestained Protein Ladder 10-250K - Pierce
     7  +1 2014 aug 27gst pull down bsaPierce GST , His and Biotin Pull - Down Kits - FAQQ. After I perform the pull - down of my prey protein, do I recover it free of the bait ... mg GST /ml; 6xHis 12-25 mg 6xHis protein/ml; biotin 9 mg biotinylated BSA /ml.
     7  -1 2014 aug 19clontech cobalt resinHisPur Cobalt Superflow Agarose - Pierce Biotechnology, Inc.HisPur Cobalt Superflow Agarose is a highly crosslinked, durable resin that ... of Thermo Scientific HisPur Cobalt Superflow Agarose or Clontech ™ TALON™ ...
     7  +15 2014 aug 12protein concentration calculatorProtein Assay Data Analysis - PierceA review of the principles, methods, and calculations of protein assay data analysis, including standard curve fitting, concentration calculation and the ...
     7  +1 2014 aug 02peptides synthesis price
     7  ~ 2014 jul 20cell distruption kitsSolution-based Cell Lysis ; Techniques of Protein Extraction by Physical Disruption. Mechanical ... P-PER Plant Protein Extraction Reagent Kit . Additives and ...
     7  ~ 2014 jul 18site x icamMouse Soluble ICAM -1 (CD54) ELISA Kits - PierceAssay sICAM-1 (mouse soluble ICAM -1, also called CD54) levels in mouse serum,
     8  +3 2014 oct 03tandem mass spectrometryOverview of Mass Spectrometry - Pierce Biotechnology, Inc.) offers further information about specific ions. In this approach, distinct ions of interest are selected based ...
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